Coding

Part:BBa_K3195004:Design

Designed by: Shan Baihui   Group: iGEM19_SEU-Nanjing-China   (2019-10-14)


Trx-His-EK tagged subtilisin-like protease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 447
    Illegal XhoI site found at 1645
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 889
    Illegal AgeI site found at 1120
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 967


Design Notes

It is the coding sequence of subtilisin-like protease found in Branchiostoma. We add 6 his tag at its tail, besides we optimized its condons for better prokaryotic expression.

Source

subtilisin-like protease gene from Branchiostoma belcheri tsingtauense

References

[1]He Chunpeng, Han Tingyu, Liao Xin, Zhou Yuxin, Wang Xiuqiang, Guan Rui, Tian Tian, Li Yixin, Bi Changwei, Lu Na, He Ziyi, Hu Bing, Zhou Qiang, Hu Yue, Lu Zuhong and Chen J.-Y. Phagocytic intracellular digestion in amphioxus (Branchiostoma)285Proc. R. Soc. B http://doi.org/10.1098/rspb.2018.0438